Figure 2.
Figure 2. Effect of multicopy Rom1 on methionine-induced internalization of Mup1-pHluorin in adaptor mutant cells. (A) WT, 4Δ+Ent1, and 4Δ+ENTH1 cells with empty vector or Rom1 as indicated were grown in the absence of methionine to promote accumulation of Mup1 at the plasma membrane. Random fields of cells were imaged before treatment (−Met) or 30 min after addition of 20 µg/ml methionine (+Met) to the medium. For all images, identical maximum and minimum intensity values were applied to allow comparison of fluorescence intensity. Bar, 2.5 µm. (B) Quantification of Mup1-pHluorin intensity (a.u.; error bars indicate mean ± SEM; ***, P < 0.001) from the experiment in A. Black bars represent untreated (−Met) cells, whereas gray bars represent methionine-treated cells (+Met).

Effect of multicopy Rom1 on methionine-induced internalization of Mup1-pHluorin in adaptor mutant cells. (A) WT, 4Δ+Ent1, and 4Δ+ENTH1 cells with empty vector or Rom1 as indicated were grown in the absence of methionine to promote accumulation of Mup1 at the plasma membrane. Random fields of cells were imaged before treatment (−Met) or 30 min after addition of 20 µg/ml methionine (+Met) to the medium. For all images, identical maximum and minimum intensity values were applied to allow comparison of fluorescence intensity. Bar, 2.5 µm. (B) Quantification of Mup1-pHluorin intensity (a.u.; error bars indicate mean ± SEM; ***, P < 0.001) from the experiment in A. Black bars represent untreated (−Met) cells, whereas gray bars represent methionine-treated cells (+Met).

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