Figure 1.
Figure 1. Suppression of temperature-dependent growth and endocytic defects by the Rho1 pathway. (A) Schematic of Rho1 pathway components. (B) Five-fold serial dilutions of WT, 4Δ+Ent1, and 4Δ+ENTH1 cells with empty vector, YAP1801, WSC1, MID2, ROM1, and RHO1 plasmids as indicated were grown at 30°C or 35.5°C. Growth at 35.5°C was scored as strong (++), moderate (+), or weak (−/+). (C) Cells from B, which express Ste3-GFP at endogenous levels, were examined by fluorescence microscopy. Insets show additional cells at the same magnification. Bar, 2.5 µm. (D) Intensity of Ste3-pHluorin was quantified in WT, 4Δ+Ent1, and 4Δ+ENTH1 cells expressing Ste3-pHluorin with the same plasmids used in B. Intensity values corrected for cell size are expressed in arbitrary units (a.u.; error bars indicate mean ± SEM; ***, P < 0.001 compared with all other conditions).

Suppression of temperature-dependent growth and endocytic defects by the Rho1 pathway. (A) Schematic of Rho1 pathway components. (B) Five-fold serial dilutions of WT, 4Δ+Ent1, and 4Δ+ENTH1 cells with empty vector, YAP1801, WSC1, MID2, ROM1, and RHO1 plasmids as indicated were grown at 30°C or 35.5°C. Growth at 35.5°C was scored as strong (++), moderate (+), or weak (−/+). (C) Cells from B, which express Ste3-GFP at endogenous levels, were examined by fluorescence microscopy. Insets show additional cells at the same magnification. Bar, 2.5 µm. (D) Intensity of Ste3-pHluorin was quantified in WT, 4Δ+Ent1, and 4Δ+ENTH1 cells expressing Ste3-pHluorin with the same plasmids used in B. Intensity values corrected for cell size are expressed in arbitrary units (a.u.; error bars indicate mean ± SEM; ***, P < 0.001 compared with all other conditions).

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