Figure 2. Cytokinesis defects in cells with mutant cofilins. Comparison of wild-type (adf1+), adf1-M2, and adf1-M3 cells. (A) DIC images at 25°C. (B) Fixed cells stained with DAPI showing many mutant cells with two nuclei. (C) Histograms of the fractions of cells with two or more nuclei in samples of >300 cells of each strain grown at 25 or 30°C in two separate experiments, fixed and stained with DAPI. (D) Fixed cells stained with Bodipy-phallacidin showing thick bundles of actin filaments in the middle of many mutant cells (arrows). Bars, 10 µm.
Figure 2.

Cytokinesis defects in cells with mutant cofilins. Comparison of wild-type (adf1+), adf1-M2, and adf1-M3 cells. (A) DIC images at 25°C. (B) Fixed cells stained with DAPI showing many mutant cells with two nuclei. (C) Histograms of the fractions of cells with two or more nuclei in samples of >300 cells of each strain grown at 25 or 30°C in two separate experiments, fixed and stained with DAPI. (D) Fixed cells stained with Bodipy-phallacidin showing thick bundles of actin filaments in the middle of many mutant cells (arrows). Bars, 10 µm.

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