H2AV is removed by stage 5 of oogenesis. (A) In spn-A¹ mutants, γ-H2AV foci are not observed past stage 4 of oogenesis. Arrows point to the oocytes. (B) In wild type (WT), an antibody that recognizes both phosphorylated and unphosphorylated versions of H2AV showed abundant staining in germarium cells until stage 3 of oogenesis and was then drastically reduced in egg chambers at stages 4 and 5. The somatic-derived follicle cells surrounding the egg chambers show strong H2AV labeling at all stages. Bars, 5 µm. (C) A graph presenting the relative H2AV fluorescence intensity of wild-type oocytes through oogenesis. The mean fluorescence intensity of H2AV was calculated for oocytes from each stage of two complete ovarioles. The error bars denote the SD.