ATM and ATR are redundant for the phosphorylation of H2AV in response to meiotic DSBs. (A and B) At the permissive temperature (18°), mei-41^D3;tefu⁸ displayed γ-H2AV foci in region 2a and 3 oocytes, similar to mei-41^D3 single mutants. (C and D) At the restrictive temperature (25°) for 1 d, no γ-H2AV staining was observed in mei-41^D3;tefu⁸ double mutant oocytes. (E and F) When the mei-41^D3;tefu⁸ double mutants were transferred from restrictive temperature back to permissive temperature for 1 d, γ-H2AV staining returned. The staining is less punctate than normal probably because H2AV phosphorylation needs time to accumulate and appear as foci (not depicted; Mehrotra and McKim, 2006). The short time span between the return to permissive conditions and fixation was necessary to accurately stage the oocytes. Bar, 5 µM.