Figure 2.
Figure 2. tefu and mei-41 are required for DSB repair in the oocyte. Oocytes were identified with an antibody against the synaptonemal complex component C(3)G. (A) γ-H2AV labeling from a wild-type (WT) germarium, showing foci in region 2a pachytene cells in which meiotic DSBs are induced. (B) In region 3 pachytene oocytes, γ-H2AV labeling is absent from wild-type germaria, indicating that DSBs have been repaired. (C and D) In a repair-defective mutant spn-A1, γ-H2AV staining persists in region 3 oocytes. (E and F) In a mei-41D3 mutant, γ-H2AV staining persists in region 3 oocytes as distinct foci (see insets). (G and H) In a tefu8 mutant, γ-H2AV staining in region 3 oocytes is in threads instead of distinct foci (see insets). (I and J) γ-H2AV is eliminated in mei-W684572;tefu8 double mutant. (K and L) Region 2a nurse cells in a tefu8 mutant have more γ-H2AV foci than wild type. Bar, 5 µm.

tefu and mei-41 are required for DSB repair in the oocyte. Oocytes were identified with an antibody against the synaptonemal complex component C(3)G. (A) γ-H2AV labeling from a wild-type (WT) germarium, showing foci in region 2a pachytene cells in which meiotic DSBs are induced. (B) In region 3 pachytene oocytes, γ-H2AV labeling is absent from wild-type germaria, indicating that DSBs have been repaired. (C and D) In a repair-defective mutant spn-A1, γ-H2AV staining persists in region 3 oocytes. (E and F) In a mei-41D3 mutant, γ-H2AV staining persists in region 3 oocytes as distinct foci (see insets). (G and H) In a tefu8 mutant, γ-H2AV staining in region 3 oocytes is in threads instead of distinct foci (see insets). (I and J) γ-H2AV is eliminated in mei-W684572;tefu8 double mutant. (K and L) Region 2a nurse cells in a tefu8 mutant have more γ-H2AV foci than wild type. Bar, 5 µm.

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