Figure 5. Hsp42 does not affect the localization of amyloidogenic aggregates. (A) RNQ1-YFP (green) was expressed in WT and hsp42Δ cells. Nuclei were visualized by coexpressing HTB1-mCherry (red). (B) WT cells expressing RNQ1-mCherry (green) were incubated at 30°C and shifted to 37°C for 30 min or 180 min (+MG132). The localization of Hsp42 (red) was determined by immunofluorescence (IF) using Hsp42-specific antibodies. Nuclei were visualized by DAPI staining (blue). The dashed lines indicate the border of respective yeast cells. Bars, 1 µm.
Figure 5.

Hsp42 does not affect the localization of amyloidogenic aggregates. (A) RNQ1-YFP (green) was expressed in WT and hsp42Δ cells. Nuclei were visualized by coexpressing HTB1-mCherry (red). (B) WT cells expressing RNQ1-mCherry (green) were incubated at 30°C and shifted to 37°C for 30 min or 180 min (+MG132). The localization of Hsp42 (red) was determined by immunofluorescence (IF) using Hsp42-specific antibodies. Nuclei were visualized by DAPI staining (blue). The dashed lines indicate the border of respective yeast cells. Bars, 1 µm.

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