Figure 6. TPX2–Eg5 interaction regulates motor function. (A) Defective spindles in cells overexpressing CT-35–GFP and stained for α-tubulin and DNA. (B) Spindle lengthening in LLC-Pk1 cells microinjected with GST or GST–CT-35. Cells are expressing EGFP–α-tubulin. (C) Spindle length for the indicated conditions (GST alone, n = 6; CC1, n = 13; GST–CT-35, n = 21; uninjected parental, n = 21; error bars represent SD). *, P > 0.05; **, P < 0.05. (D) Eg5 and TPX2 localize to midzone microtubules by TIRF microscopy. (left) Overlay of Eg5LAP visualized by widefield (green) and by TIRF (red). (right) Overlay of TPX2LAP imaged in TIRF and mCherry microtubules in widefield (green). Bars, 5 µm.
Figure 6.

TPX2–Eg5 interaction regulates motor function. (A) Defective spindles in cells overexpressing CT-35–GFP and stained for α-tubulin and DNA. (B) Spindle lengthening in LLC-Pk1 cells microinjected with GST or GST–CT-35. Cells are expressing EGFP–α-tubulin. (C) Spindle length for the indicated conditions (GST alone, n = 6; CC1, n = 13; GST–CT-35, n = 21; uninjected parental, n = 21; error bars represent SD). *, P > 0.05; **, P < 0.05. (D) Eg5 and TPX2 localize to midzone microtubules by TIRF microscopy. (left) Overlay of Eg5LAP visualized by widefield (green) and by TIRF (red). (right) Overlay of TPX2LAP imaged in TIRF and mCherry microtubules in widefield (green). Bars, 5 µm.

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