Spindle localization of Eg5 is dependent on an interaction with TPX2. (A) Distribution of Eg5 and α-tubulin in parental cells and parental cells expressing no transgene (none) or the indicated transgene and treated with siRNA-targeting endogenous pig TPX2. (B and C) Corresponding fluorescence intensity profiles of cells in A (bottom two rows). (D) Ratio of Eg5/tubulin fluorescence intensity (n > 20). Error bars represent SD. **, P < 0.01. (E) Distribution of TPX2 and α-tubulin in parental (control) or cells depleted of Eg5 or treated with monastrol for 1 or 6 h. (F) Ratio of TPX2/tubulin fluorescence intensity (n > 20). Error bars represent SD. **, P < 0.01. Bars, 5 µm.