Disorganized spindles and spindle pole fragmentation in cells lacking TPX2–Eg5 interaction. (A and B) Selected frames from time-lapse imaging of cells expressing mCherry α-tubulin and either mTPX2^LAP (A) or mTPX2-710^LAP (B) and treated with siRNA-targeting pig TPX2. (B, top row) Clusters of microtubules moving away from spindle poles, forming extra foci (arrows). (middle row) Elongation of microtubule bundles in the spindle midzone. (bottom row) Microtubule nucleation near chromosomes (arrows). Bars, 5 µm.