Figure 5. Effect of the inhibitors on... | Rockefeller University Press

Figure 5.

$Figure 5. Effect of the inhibitors on the distribution of in vivo–transcribed U snRNAs. A mixture of the plasmids harboring the U1ΔSm and U2ΔSm genes was microinjected into the nuclei of Xenopus oocytes either alone (lanes 1–3) or together with control (ctr) IgG (lanes 4–6), anti-PHAX antibody (lanes 7–9), ΔCD protein (lanes 10–12), ΔN protein (lanes 13–15), or ΔNES protein (lanes 16–18), and the nucleocytoplasmic distribution of the transcripts was analyzed by Northern blotting of the Nsup, Nppt, and cytoplasmic (C) RNA fractions after 1.5 h. Black lines indicate that intervening lanes have been spliced out. (B and C) The ratio of the Nppt signal against the nuclear fraction total signal of U1ΔSm and U2ΔSm, respectively, was calculated from three independent experiments as in A. The means and the standard deviations for WGA+ctr IgG, WGA+anti-PHAX antibody, WGA+ΔCD, WGA+ΔN, and WGA+ΔNES are shown.$

Effect of the inhibitors on the distribution of in vivo–transcribed U snRNAs. A mixture of the plasmids harboring the U1ΔSm and U2ΔSm genes was microinjected into the nuclei of Xenopus oocytes either alone (lanes 1–3) or together with control (ctr) IgG (lanes 4–6), anti-PHAX antibody (lanes 7–9), ΔCD protein (lanes 10–12), ΔN protein (lanes 13–15), or ΔNES protein (lanes 16–18), and the nucleocytoplasmic distribution of the transcripts was analyzed by Northern blotting of the Nsup, Nppt, and cytoplasmic (C) RNA fractions after 1.5 h. Black lines indicate that intervening lanes have been spliced out. (B and C) The ratio of the Nppt signal against the nuclear fraction total signal of U1ΔSm and U2ΔSm, respectively, was calculated from three independent experiments as in A. The means and the standard deviations for WGA+ctr IgG, WGA+anti-PHAX antibody, WGA+ΔCD, WGA+ΔN, and WGA+ΔNES are shown.

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