Figure 5. Loss of subapical actin results in a loss of metachronal cilia beating. (A) A cell from a polarized embryo treated with Cyto D with centrin-RFP and GFP-CLAMP and stained with phalloidin. The dotted line indicates the cross section in A’. (B) Circular diagrams showing mean cilia orientation from polarized embryos treated at stage 29 with either 0.1% DMSO (top) or 10 µM Cyto D (bottom) for 12 h before fixation. (C) Kymographs at cilia tips from videos of cilia beating from 0.1% DMSO (top; Video 2) and 10 µM Cyto D–treated embryos (bottom; Video 3). (D) A diagram of the high degree of polarized cytoskeletal organization in ciliated cells and the proposed model for the propagation of a physical signal involved in metachronal synchrony.
Figure 5.

Loss of subapical actin results in a loss of metachronal cilia beating. (A) A cell from a polarized embryo treated with Cyto D with centrin-RFP and GFP-CLAMP and stained with phalloidin. The dotted line indicates the cross section in A’. (B) Circular diagrams showing mean cilia orientation from polarized embryos treated at stage 29 with either 0.1% DMSO (top) or 10 µM Cyto D (bottom) for 12 h before fixation. (C) Kymographs at cilia tips from videos of cilia beating from 0.1% DMSO (top; Video 2) and 10 µM Cyto D–treated embryos (bottom; Video 3). (D) A diagram of the high degree of polarized cytoskeletal organization in ciliated cells and the proposed model for the propagation of a physical signal involved in metachronal synchrony.

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