Figure 2. Cyto D and Noc treatment affects the generation of robust fluid flow but does not affect cilia structure or beat frequency. (A–C) Cyto D (A)–, mock (B)-, or Noc (C)-treated embryos stained with β-tubulin and phalloidin. Apical (A′) and subapical (A″) staining of actin with phalloidin of an embryo treated with 10 µM Cyto D. (B′ and C′) Images of maximum projection time-lapse videos acquired every 2 s over a 30-s time period of CLIP170-expressing embryos from control (B′) or Noc-treated cells (C′). (D) Quantification of flow velocity for embryos treated between stages 23 and 29 with mock, Cyto D, or Noc (n = 50, 10 flow lines from five embryos for each condition). (E) Quantification of the mean beat frequency of embryos treated between stages 23 and 29 with mock, Cyto D, or Noc (n = 75, five cilia from three individual cells on five different embryos for each condition). All error bars represent the SD of the mean.
Figure 2.

Cyto D and Noc treatment affects the generation of robust fluid flow but does not affect cilia structure or beat frequency. (A–C) Cyto D (A)–, mock (B)-, or Noc (C)-treated embryos stained with β-tubulin and phalloidin. Apical (A′) and subapical (A″) staining of actin with phalloidin of an embryo treated with 10 µM Cyto D. (B′ and C′) Images of maximum projection time-lapse videos acquired every 2 s over a 30-s time period of CLIP170-expressing embryos from control (B′) or Noc-treated cells (C′). (D) Quantification of flow velocity for embryos treated between stages 23 and 29 with mock, Cyto D, or Noc (n = 50, 10 flow lines from five embryos for each condition). (E) Quantification of the mean beat frequency of embryos treated between stages 23 and 29 with mock, Cyto D, or Noc (n = 75, five cilia from three individual cells on five different embryos for each condition). All error bars represent the SD of the mean.

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