Figure 3. The Pp1-87B phosphatase down-regulates Moe activation. (A) Protein extracts from S2 cells treated with Calyculin A (Caly. A) or dsRNAs targeting Slik and the catalytic subunits of each Drosophila PP1 and PP2A phosphatases were analyzed by Western blotting using antibodies against Moe (bottom) or specific for T559-phosphorylated Moe (P-Moe; top). The same blot is shown at two different expositions (exp.) for P-Moe. flw, Flapwing; mts, Microtubule star. (B) Immunodetection of P-Moe in control S2 cells or after depletion of Slik or Pp1-87B. (C) Dynamics of Moe-GFP during the cell cycle after depletion of Pp1-87B. Arrowheads show persistence of the association of Moe-GFP with the polar cortex. (D) Distribution of GFP–Pp1-87B (top) and Slik-GFP (bottom). Division stages were identified with α-Tubulin-mCherry (not depicted). Bars, 10 µm.
Figure 3.

The Pp1-87B phosphatase down-regulates Moe activation. (A) Protein extracts from S2 cells treated with Calyculin A (Caly. A) or dsRNAs targeting Slik and the catalytic subunits of each Drosophila PP1 and PP2A phosphatases were analyzed by Western blotting using antibodies against Moe (bottom) or specific for T559-phosphorylated Moe (P-Moe; top). The same blot is shown at two different expositions (exp.) for P-Moe. flw, Flapwing; mts, Microtubule star. (B) Immunodetection of P-Moe in control S2 cells or after depletion of Slik or Pp1-87B. (C) Dynamics of Moe-GFP during the cell cycle after depletion of Pp1-87B. Arrowheads show persistence of the association of Moe-GFP with the polar cortex. (D) Distribution of GFP–Pp1-87B (top) and Slik-GFP (bottom). Division stages were identified with α-Tubulin-mCherry (not depicted). Bars, 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal