Moe controls polar relaxation and retraction of short-lived blebs. (A) Rapid time-lapse imaging of a cell line coexpressing Moe-GFP and α-Tubulin-mCherry in control condition (top) and after Slik depletion (bottom). The merge image shows maximum projection and close-up single focal planes from the boxed region; Moe-GFP is shown in black. Arrowhead shows an apparent cortical rupture at the site of bleb formation, and arrows point to unregulated blebs. (B) Individual blebs were plotted according to their maximal length in controls cells and after Slik or Moe dsRNA depletion. Bars represent mean values for each condition. (C) Rapid time-lapse imaging of a GFP-Utrophin-CH (GFP-UtrCH; F-actin probe) and α-Tubulin-mCherry cell line in control conditions (top) and after Moe or Slik depletion. Close-ups correspond to the framed region and show the GFP signal in black. Arrowheads show cortical actin rupture and F-actin defective recruitment to the bleb membrane. Bars, 10 µm.