Control of Moe activation contributes to cell elongation and cytokinesis. (A) Time lapse of Moe distribution throughout the cell cycle observed in a stable S2 cell line coexpressing Moe-GFP (white) and α-Tubulin-mCherry (not depicted). (B) Dynamics of Moe-GFP during anaphase cell elongation. Top images show a single focal plane of Moe-GFP, and bottom images show maximum projections of Moe-GFP and α-Tubulin-mCherry. (C) Quantification of the equatorial enrichment of Moe-GFP (green) during anaphase elongation (blue) measured as the ratio between cell length along the spindle (L) versus the equator (I). (D) Dynamics of Moe-TD-GFP as observed in living cells upon depletion of endogenous Moe. Moe-TD-GFP remained associated with the polar cortex in anaphase (arrowheads) and caused binucleation (asterisks point to nuclei). (E) Distribution of the maximal elongation of individual Moe-GFP or Moe-TD-GFP cells (cell length in anaphase B/cell length in metaphase). Black dots represent cells that successfully passed through division, and red dots indicate cytokinesis failure. Bars represent mean values for each condition. (F) F-actin distribution in cells expressing Moe-GFP or Moe-GFP-TD upon depletion of endogenous Moe. The right-most images show maximum projection (Max. Proj.). (G and H) Polar enrichment of Moe (G) and F-actin (H) in cells expressing Moe-GFP or Moe-TD-GFP in the absence of endogenous Moe. Boxes show top and bottom quartiles, horizontal lines show median values, and vertical lines show minimal and maximal values. Bars, 10 µm.