CLEM and rapid-freeze images of rodlike aggregates in [PSI⁺] cells treated with GuHCl. (A–C) Fluorescence (A) and EM (B and C) images of a rodlike Sup35NM-GFP aggregate in a [PSI⁺] cell treated with GuHCl for 15 h. Cells expressing Sup35NM-GFP were fixed after fluorescence imaging (A), and the thin section containing the same rodlike aggregate identified by the fluorescence microscopy was subjected to EM analysis (B). (C) Magnified image of the boxed area in B. (D) Rapid-freeze EM images of a rodlike aggregate in a [PSI⁺] cell treated with GuHCl. The boxed areas in the left image are magnified on the right. (E and F) Immunogold labeling of the thin-section EM images of aggregates in [PSI⁺] cells treated with GuHCl. An anti-GFP antibody was used to attach the gold.