Figure 7. Set7 is required for skeletal muscle development and myofibril assembly. Zebrafish embryos were injected with control (CTRL) or Set7 morpholino (MO) at the single-cell stage, and embryos were collected 24 h after fertilization. (A) RT-PCR analysis of zebrafish Set7 transcripts from control and Set7 morpholino embryos. Mutant (Mut) and wild-type (Wt) transcripts are indicated. (B) Bright-field embryonic images of control and Set7 morpholino. (C) Set7 morphants display defects in myofibril assembly as indicated by MHC immunostaining. Higher magnification is shown on the right. DAPI counter stains the nucleus. Bars, 60 µm. (D) Set7 morphants display defects in fast MHC expression. (E) Set7 morphants display defects in slow MHC expression. (F) MEF2 protein expression level was significantly decreased in Set7 morphants. Bar, 30 µm.
Figure 7.

Set7 is required for skeletal muscle development and myofibril assembly. Zebrafish embryos were injected with control (CTRL) or Set7 morpholino (MO) at the single-cell stage, and embryos were collected 24 h after fertilization. (A) RT-PCR analysis of zebrafish Set7 transcripts from control and Set7 morpholino embryos. Mutant (Mut) and wild-type (Wt) transcripts are indicated. (B) Bright-field embryonic images of control and Set7 morpholino. (C) Set7 morphants display defects in myofibril assembly as indicated by MHC immunostaining. Higher magnification is shown on the right. DAPI counter stains the nucleus. Bars, 60 µm. (D) Set7 morphants display defects in fast MHC expression. (E) Set7 morphants display defects in slow MHC expression. (F) MEF2 protein expression level was significantly decreased in Set7 morphants. Bar, 30 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal