Figure 8. Pak1TE expression rescues myosin IIA distribution at a high adhesion strength. (A) pMLC immunofluorescence and F-actin phalloidin staining in migrating PtK1 cells plated on high FN concentration (30 µg/ml) and expressing GFP (noted FN30 control) or GFP–Pak1TE (noted FN30 Pak1). Insets in pMLC column show GFP expression of the GFP or GFP-Pak1TE constructs. Red lines indicate the leading edge of the cells as determined by the F-actin staining. White boxes in merge column indicate the positions of insets for higher magnifications shown in the rightmost column. (B) Fluorescence intensity of pMLC and F-actin for each indicated condition, measured from the cell edge (0 µm) into the cell center (10 µm). The data shown represent one experiment and are averaged from n ≥ 7 cells for each condition. The experiment was repeated at least three times with similar results. The arrows indicate the myosin IIA–depleted zone from the cell edge.
Figure 8.

Pak1TE expression rescues myosin IIA distribution at a high adhesion strength. (A) pMLC immunofluorescence and F-actin phalloidin staining in migrating PtK1 cells plated on high FN concentration (30 µg/ml) and expressing GFP (noted FN30 control) or GFP–Pak1TE (noted FN30 Pak1). Insets in pMLC column show GFP expression of the GFP or GFP-Pak1TE constructs. Red lines indicate the leading edge of the cells as determined by the F-actin staining. White boxes in merge column indicate the positions of insets for higher magnifications shown in the rightmost column. (B) Fluorescence intensity of pMLC and F-actin for each indicated condition, measured from the cell edge (0 µm) into the cell center (10 µm). The data shown represent one experiment and are averaged from n ≥ 7 cells for each condition. The experiment was repeated at least three times with similar results. The arrows indicate the myosin IIA–depleted zone from the cell edge.

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