Figure 1. The expression of Set7 is increased during skeletal muscle differentiation. (A) RT-PCR analyses of Set7 expression using RNAs isolated from adult mouse tissues as indicated. GAPDH was used as a loading control. (B) RT-PCR analyses of the expression of Set7 and skeletal muscle (Sk muscle) α-actin (SK-α-Actin) using total RNA isolated from C2C12 myoblasts cultured in differentiation medium for 0, 1, 3, 5, 7, and 10 d (D0–D10). GAPDH was used as a loading control. (C) C2C12 myoblasts were cultured in differentiation medium for 0, 1, 3, 5, 7, and 10 d. Western blot analyses were performed with cell extracts using antibodies that recognize the indicated proteins. β-Tubulin was used as a loading control.
Figure 1.

The expression of Set7 is increased during skeletal muscle differentiation. (A) RT-PCR analyses of Set7 expression using RNAs isolated from adult mouse tissues as indicated. GAPDH was used as a loading control. (B) RT-PCR analyses of the expression of Set7 and skeletal muscle (Sk muscle) α-actin (SK-α-Actin) using total RNA isolated from C2C12 myoblasts cultured in differentiation medium for 0, 1, 3, 5, 7, and 10 d (D0–D10). GAPDH was used as a loading control. (C) C2C12 myoblasts were cultured in differentiation medium for 0, 1, 3, 5, 7, and 10 d. Western blot analyses were performed with cell extracts using antibodies that recognize the indicated proteins. β-Tubulin was used as a loading control.

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