The role of WASH and actin in lysosome neutralization. A schematic diagram of the model proposed in this paper: (a) WASH recruited to acidic lysosomes; (b) WASH puncta cause local actin polymerization, and F-actin binds to V-ATPase; (c) small recycling vesicles bud off, and V-ATPase is recruited by WASH/F-actin; (d) loss of V-ATPase causes the vesicle to neutralize and mature to postlysosome; (e) WASH is removed some time after neutralization.