Figure 1.
Figure 1. WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved widefield images of parent (AX2) and WASH-null (wshA−) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental (n = 82) and wshA− (n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA− cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA− cells. Bars, 10 µm.

WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved widefield images of parent (AX2) and WASH-null (wshA−) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental (n = 82) and wshA− (n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA− cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA− cells. Bars, 10 µm.

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