Figure 7. Bub1 overexpression accelerates Myc-mediated lymphomagenesis. (A) Human Bub1 gene transcript was measured from a panel of 59 primary human tumors and normal peripheral B cells using qRT-PCR and normalized to TBP. The difference between lymphomas and normal peripheral B cells was statistically significant (P < 0.05) for all groups, except chronic lymphocytic leukemia. (B) Protein extracts from the indicated lymphoma derived cell lines were immunoblotted for Bub1, Aurora B, pCenp-A, pT121-H2A, and pS10-H3. (C) Survival curves for Eμ-Myc and Eμ-Myc/Bub1T85 mice. *, P < 0.05 vs. Eμ-Myc mice (log-rank test). (D) Quantification of chromosome 4 and 7 copies in EµMyc and Eμ-Myc/Bub1T85 B cell lymphoma cells. *, P < 0.05 (unpaired t test) FISH signals of 100 cells were per lymphoma. Seven lymphomas were analyzed per genotype. Error bars represent SEM.
Figure 7.

Bub1 overexpression accelerates Myc-mediated lymphomagenesis. (A) Human Bub1 gene transcript was measured from a panel of 59 primary human tumors and normal peripheral B cells using qRT-PCR and normalized to TBP. The difference between lymphomas and normal peripheral B cells was statistically significant (P < 0.05) for all groups, except chronic lymphocytic leukemia. (B) Protein extracts from the indicated lymphoma derived cell lines were immunoblotted for Bub1, Aurora B, pCenp-A, pT121-H2A, and pS10-H3. (C) Survival curves for Eμ-Myc and Eμ-Myc/Bub1T85 mice. *, P < 0.05 vs. Eμ-Myc mice (log-rank test). (D) Quantification of chromosome 4 and 7 copies in EµMyc and Eμ-Myc/Bub1T85 B cell lymphoma cells. *, P < 0.05 (unpaired t test) FISH signals of 100 cells were per lymphoma. Seven lymphomas were analyzed per genotype. Error bars represent SEM.

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