Figure 2. Vinculin is recruited to active cell–cell junctions in a myosin-dependent manner. (A) IF after CSK buffer extraction shows HGF-induced and myosin II–dependent α-catenin and vinculin distribution. (B) Magnified view of vinculin in cell–cell junctions of HGF-stimulated cells. (C) Cells expressing EGFP-vinculin (stably) and mCherry–p120-catenin (p120-ctn; transiently) analyzed by widefield and TIRF microscopy 1 h after HGF treatment. 3 µM ML-7 and 10 µM Y27632 were added 15 min after the start of imaging and washed out after another 10 min. EGFP-vinculin fluorescence intensity was measured in FAs (15 ROIs, each containing two to four FAs from seven time lapses) and cell–cell contacts (eight ROIs from seven time lapses).
Figure 2.

Vinculin is recruited to active cell–cell junctions in a myosin-dependent manner. (A) IF after CSK buffer extraction shows HGF-induced and myosin II–dependent α-catenin and vinculin distribution. (B) Magnified view of vinculin in cell–cell junctions of HGF-stimulated cells. (C) Cells expressing EGFP-vinculin (stably) and mCherry–p120-catenin (p120-ctn; transiently) analyzed by widefield and TIRF microscopy 1 h after HGF treatment. 3 µM ML-7 and 10 µM Y27632 were added 15 min after the start of imaging and washed out after another 10 min. EGFP-vinculin fluorescence intensity was measured in FAs (15 ROIs, each containing two to four FAs from seven time lapses) and cell–cell contacts (eight ROIs from seven time lapses).

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