Figure 6. Tbx3-dependent E-cadherin expression whose decrease leads to increased cadherin-6. (A and B) Tbx3-dependent E-cadherin expression in MDCK cells and Tara-KD cells. Mouse S692 of Tbx3 (outlined in red) was the predicted phosphorylation site, based on its conservation with S675 of mouse Tbx2. As shown in immunoblotting of stable clones (A) and immunofluorescence micrographs of transient transfectants (with green nuclei, B), when exogenous wild-type Tbx3 was expressed in MDCK cells, the E-cadherin expression was decreased, and the cadherin-6 expression increased concomitantly. The dephosphomimic Tbx3 (Tbx3-S692A) showed no inhibition of E-cadherin expression (A). When exogenous wild-type Tbx3 was expressed in MDCK cells, the E-cadherin expression was decreased, and the cadherin-6 expression increased concomitantly. The dephosphomimic Tbx3 (Tbx3-S692A) showed no inhibition of E-cadherin expression. The dephosphomimetic Tbx3 suppressed the down-regulation of E-cadherin and the up-regulation of cadherin-6 in Tara-KD cells. Densitometric quantification of Western blot bands was performed using Photoshop 7.0 (Adobe). As to the “Relative Intensity,” the ratio of intensities of E-cadherin to α-tubulin and cadherin-6 to α-tubulin in control were normalized to 1.0. The results are expressed as means ± SD (error bars) and are representative of three independent experiments, in which antigens were immunoblotted in the same membranes. **, P < 0.01; *, P < 0.05. The transfected cells are the ones with green nuclei. Bar, 10 µm. (C) Knockdown of E-cadherin in MDCK cells. E-cadherin, red; cadherin-6, green (cy5-staining). Bar, 10 µm. When the immunofluorescent signals of E-cadherin were decreased by E-cadherin-KD in MDCK cells, those for cadherin-6 increased.
Figure 6.

Tbx3-dependent E-cadherin expression whose decrease leads to increased cadherin-6. (A and B) Tbx3-dependent E-cadherin expression in MDCK cells and Tara-KD cells. Mouse S692 of Tbx3 (outlined in red) was the predicted phosphorylation site, based on its conservation with S675 of mouse Tbx2. As shown in immunoblotting of stable clones (A) and immunofluorescence micrographs of transient transfectants (with green nuclei, B), when exogenous wild-type Tbx3 was expressed in MDCK cells, the E-cadherin expression was decreased, and the cadherin-6 expression increased concomitantly. The dephosphomimic Tbx3 (Tbx3-S692A) showed no inhibition of E-cadherin expression (A). When exogenous wild-type Tbx3 was expressed in MDCK cells, the E-cadherin expression was decreased, and the cadherin-6 expression increased concomitantly. The dephosphomimic Tbx3 (Tbx3-S692A) showed no inhibition of E-cadherin expression. The dephosphomimetic Tbx3 suppressed the down-regulation of E-cadherin and the up-regulation of cadherin-6 in Tara-KD cells. Densitometric quantification of Western blot bands was performed using Photoshop 7.0 (Adobe). As to the “Relative Intensity,” the ratio of intensities of E-cadherin to α-tubulin and cadherin-6 to α-tubulin in control were normalized to 1.0. The results are expressed as means ± SD (error bars) and are representative of three independent experiments, in which antigens were immunoblotted in the same membranes. **, P < 0.01; *, P < 0.05. The transfected cells are the ones with green nuclei. Bar, 10 µm. (C) Knockdown of E-cadherin in MDCK cells. E-cadherin, red; cadherin-6, green (cy5-staining). Bar, 10 µm. When the immunofluorescent signals of E-cadherin were decreased by E-cadherin-KD in MDCK cells, those for cadherin-6 increased.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal