Disruption of centrobin–tubulin interaction inhibits centrobin recruitment to the centriole biogenesis site and centriole elongation. (A and B) Centrobin-TuBD overexpression blocks centrobin recruitment to the centrioles.HeLa cells transfected with control or GFP–centrobin-TuBD vectors and treated with HU were stained with anti-centrobin and anti-tubulin antibodies. The percentage of cells with indicated numbers of centrobin-positive centrioles is shown in B. (C–F) Centrobin-TuBD overexpression inhibits centriole duplication and elongation. Experiments were performed as depicted in the schematic (G), except that the cells were stained with anti–centrin 3 (C and D) and CP110 (E and F) antibodies. D and F represent the percentage of cells with the indicated number of centrin 3– and CP110-positive centrioles, respectively. Histograms are plotted as mean ± SEM (n = 3). Asterisks denote that the difference is significant, and P < 0.001. Bars, 1 µm.