Figure 6.
Figure 6. Centrobin-TuBD disrupts the interaction of full-length centrobin with tubulin. (A) Overexpression of centrobin-TuBD disrupts the interaction of endogenous full-length centrobin with a-tubulin. 293T cells transfected with vector, GFP-centrobin or GFP–centrobin-TuBD, or in combination as indicated were lysed and subjected to immunoprecipitation (IP) with anti–α-tubulin antibody. The bound proteins were fractionated and detected by immunoblotting (IB) using the indicated antibodies. (B) Centrobin-TuBD does not have the potential to dimerize/polymerize with full-length centrobin. 293T cells transfected with vector, myc-centrobin, or GFP-myc–centrobin-TuBD along with GFP-centrobin–expressing constructs were lysed and subjected to immunoprecipitation using anti-myc antibody. Immunoblotting was performed with anti-GFP and anti-myc antibodies. Molecular mass is indicated in kilodaltons.

Centrobin-TuBD disrupts the interaction of full-length centrobin with tubulin. (A) Overexpression of centrobin-TuBD disrupts the interaction of endogenous full-length centrobin with a-tubulin. 293T cells transfected with vector, GFP-centrobin or GFP–centrobin-TuBD, or in combination as indicated were lysed and subjected to immunoprecipitation (IP) with anti–α-tubulin antibody. The bound proteins were fractionated and detected by immunoblotting (IB) using the indicated antibodies. (B) Centrobin-TuBD does not have the potential to dimerize/polymerize with full-length centrobin. 293T cells transfected with vector, myc-centrobin, or GFP-myc–centrobin-TuBD along with GFP-centrobin–expressing constructs were lysed and subjected to immunoprecipitation using anti-myc antibody. Immunoblotting was performed with anti-GFP and anti-myc antibodies. Molecular mass is indicated in kilodaltons.

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