Figure 4.
Centrobin interacts with α-tubulin in vivo. 293T cells transfected with control or GFP-centrobin vectors were lysed and subjected to immunoprecipitation (IP) using anti–α-tubulin antibody. Immunoblotting (IB) was performed with anti-GFP and anti–α-tubulin antibodies for detection of GFP-centrobin and endogenous α-tubulin, respectively. Molecular mass is indicated in kilodaltons.