Centrobin is recruited to the centriole biogenesis site after hSAS-6. (A [first and second panels] and B) hSAS-6 recruitment to the centriole biogenesis site is not dependent on the presence of centrobin. HeLa cells transfected with control or centrobin siRNA were treated with HU and stained with anti-centrobin, hSAS-6, and α-tubulin antibodies. The percentage of hSAS-6–positive centrioles is shown in B. (A [first and third panels] and C) Centrobin recruitment to the centriole biogenesis site is dependent on the presence of hSAS-6. HeLa cells treated with control or hSAS-6 siRNAs and HU were stained as previously described for A, first and second panels, and the percentage of cells with centrobin-positive centrioles is plotted in C. Histograms are plotted as mean ± SEM (n = 3). The asterisk denotes that the difference is significant, and P < 0.001. Bars, 1 µm.