Variable expression of Sg2 and NPY in GFAP-positive astrocytes of the human brain temporal cortex. All images are z projections of stacks ∼8 (A and B)- and 4 (A’ and A’’)-µm thick. The inset in A shows the DAPI fluorescence of the nuclei in the figure. (A–A’’) The levels of Sg2 in the GFAP-positive astrocytes vary considerably from high (arrowhead; see the zoom in A’) to low (arrow; see the zoom in A’’). (B) The triple immunolabeling of a few astrocytes identified by their positivity for GFAP that exhibit variable levels of REST and NPY (gray). Notice that the cell with high REST in the nucleus (arrows) shows a weak NPY signal in the cytoplasm, whereas two cells with lower REST (arrowheads) show higher signals for the peptide. Bars: (A and B) 30 µm; (A’ and A’’) 5 µm.