Expression of neuro/gliosecretion proteins in cultured astrocytes transfected with GFP or with the dominant-negative construct REST/DBD-GFP. (B) Representative Western blots illustrating the expression of eleven neuro/gliosecretion proteins in the two transfected astrocyte populations. (A and C) Notice that in eight of them, the levels are unaffected by the dominant-negative construct, whereas in three, Sg2, SNAP25, and Stx1a, gene expression is increased at both the mRNA (A) and the protein (C) level. Error bars indicate SEM (A) and SD (C). *, P < 0.05; **, P < 0.01. (D and E) Transfection of REST/DBD-GFP does not change the expression of three astrocyte protein markers, GS, GFAP, and S100β. Results are from three experiments performed in triplicate. Error bars indicate SD.