Quantitative analysis of 3D ER domain dimensions. (A) Nine individual wt ER tubules were measured at 50-nm intervals along their lengths. Tubule lengths range from ∼250 to 700 nm; each tubule is shown in a different color. (B) Histogram showing the distribution of all tubER diameter measurements (n = 107 measurements taken from the nine tubules shown in A). (C) As in B for cecER thickness (n = 88 measurements). (D) As in B for pmaER thickness (n = 106 measurements). (E) Comparison of mean diameters/thickness for ER domains calculated from the data points shown in B–D. Brackets show range of measurements, and boxes show SEM. Horizontal lines show means given above the boxes.