3D ER domain distribution and abundance during inheritance. (A–F) 3D models derived from 200-nm-thick section serial tomograms show ER domain organization in six different wt cells ordered by increasing bud sizes. Corresponding 2D tomographs are shown in Fig. S3 (A–F). Panels show domain distribution of all ER domains (left), cecER and tubER (yellow and green domains in middle), and pmaER alone (blue on right). (G) Graph of peripheral ER domain volumes found in reconstructed sections of cells in A–F. (H) Relative percentage of each domain in the mother cell for A–F. (I) As in G for the bud. (J) As in H for the bud ER. Bars, 200 nm.