Factors involved in hair morphogenesis are de-regulated in Runx1 cKO skin. (A) Summary of known HF morphogenesis factors (Nakamura et al., 2001) tested in C by QRT-PCR analysis of skin sorted α6-integrinmedium and α6-integrinnegative cells (n = 2 mice per genotype). *, P < 0.06; **, P < 0.006. (B) Same as C but for Runx1 mRNA. Note high expression of Runx1 in the α6-integrinmedium, which indicates enrichment in HF cells over the epidermis. (D–F) Immunofluorescence E16.5 skin images reveals reduction in Lef1 signal in cKO dermal and epidermal skin at all developmental stages analyzed. (G, I, and J) E18.5 BAT-GAL mouse skin showed reduced X-Gal staining in both epithelial and mesenchymal cKO skin when compared with WT. (H) Quantification of G (n = 2 WT and cKO; 300 HFs). (K) Quantification of Lef1 staining from images like D and E (n = 3 WT: 687 HFs and n = 3 cKOs: 248 HFs). **, P ≤ 0.007; *, P = 0.09. Epi, epidermis; Der, dermis. Bars, 10–50 µm.
