CENP-A incorporation in CAF-1 p150– and HIRA-depleted extracts. (A) Immunoblot analysis of increasing amounts of a mock-depleted extract (expressed as percentage of a 1.5-µl aliquot) and 1.5-µl aliquots of CAF-1 p150– and HIRA-depleted extracts with the indicated antibodies. RbAp48 levels are also shown. (B) Immunoblot analysis of aliquots from a mock-depleted extract, HIRA- and CAF-1 p150–depleted extracts to show the remaining levels of CENP-A. RbAp46 levels are also shown. (C) Bar graph showing the CENP-A loading efficiency in the depleted extracts with respect to the mock. Bars represent mean ± SE from two independent experiments.