Figure 8. GFP-CEACAM1 is concentrated in the primary cilium after addition of a microtubule-binding domain. (A) Live cells expressing GFP-CEACAM1-tau were imaged 4 d after seeding. The image is a projection of a z stack and was collected with identical microscope settings to Fig. 1 C. Bar, 10 µm. (B) FRAP measurements of GFP-CEACAM1-tau 4 d after seeding show mobility similar to GFP-CEACAM1. GFP-CEACAM1 data are from Fig. 5 and are included as a reference. Each FRAP value is a mean of measurements from eight cells. Error bars represent standard deviation between measurements. (C) XZ sections of cells 12 d after seeding show GFP-CEACAM1-tau enriched in the cilium relative to the apical membrane, whereas GFP-CEACAM1 is evenly distributed. Bar, 3 µm.
Figure 8.

GFP-CEACAM1 is concentrated in the primary cilium after addition of a microtubule-binding domain. (A) Live cells expressing GFP-CEACAM1-tau were imaged 4 d after seeding. The image is a projection of a z stack and was collected with identical microscope settings to Fig. 1 C. Bar, 10 µm. (B) FRAP measurements of GFP-CEACAM1-tau 4 d after seeding show mobility similar to GFP-CEACAM1. GFP-CEACAM1 data are from Fig. 5 and are included as a reference. Each FRAP value is a mean of measurements from eight cells. Error bars represent standard deviation between measurements. (C) XZ sections of cells 12 d after seeding show GFP-CEACAM1-tau enriched in the cilium relative to the apical membrane, whereas GFP-CEACAM1 is evenly distributed. Bar, 3 µm.

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