Figure 7. Smo can be retained outside the CMD by association with the retention matrix. Live cells expressing GFP-Smo-NHERF1-FR or GFP-Smo-NHERF1 were labeled with Alexa Fluor 647 anti-GFP on the apical side (surface label). (A) XY projections of cells expressing the ERM binding–deficient GFP-Smo-NHERF1-FR exhibit bright spots corresponding to cilia. Pairs of spots are concentrations of GFP at the base and tip of a single cilium. In contrast, Smo fused to a NHERF1 domain capable of binding ERMs (GFP-Smo-NHERF1) was localized over the entire apical plasma membrane outside the CMD. Bar, 10 µm. (B) XZ sections of cells expressing GFP-Smo-NHERF1-FR reveal cilia with an uneven distribution of GFP. Surface labeling shows GFP-Smo-NHERF1 across the apical membrane. Image brightness was increased in B. Bar, 5 µm.
Figure 7.

Smo can be retained outside the CMD by association with the retention matrix. Live cells expressing GFP-Smo-NHERF1-FR or GFP-Smo-NHERF1 were labeled with Alexa Fluor 647 anti-GFP on the apical side (surface label). (A) XY projections of cells expressing the ERM binding–deficient GFP-Smo-NHERF1-FR exhibit bright spots corresponding to cilia. Pairs of spots are concentrations of GFP at the base and tip of a single cilium. In contrast, Smo fused to a NHERF1 domain capable of binding ERMs (GFP-Smo-NHERF1) was localized over the entire apical plasma membrane outside the CMD. Bar, 10 µm. (B) XZ sections of cells expressing GFP-Smo-NHERF1-FR reveal cilia with an uneven distribution of GFP. Surface labeling shows GFP-Smo-NHERF1 across the apical membrane. Image brightness was increased in B. Bar, 5 µm.

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