Overexpression of Sk2 in a car1 mutant restores Rh1 and TRP levels, and photoreceptor degeneration is suppressed. (A) Rh1 and TRP levels are recovered when Sk2 is overexpressed in a car mutant. (B) The percentage of expression of Rh1 and TRP in an Sk2 overexpressor (Sk2 OE) and an Sk2 overexpressor in a car mutant background compared with car mutant alone. n = 3; bars denote standard deviation. (C) Degeneration is suppressed in 7-d-old photoreceptors of the Sk2 overexpressor in a car mutant. 4% of rhabdomeres are intact in Sk2 overexpressors, whereas 88% are intact in Sk2 overexpressors in car. (D) S2 cells were stained with antibodies to Sk1 and cadherin, a plasma membrane marker. Overlay shows the colocalization of Sk1 with cadherin. S2 cells constitutively expressing GFP-tagged Lamp1 were stained with antibodies to Sk2. Overlay shows the colocalization of Sk2 with Lamp1-GFP.
