Figure 7.

Miller spread of 190 and 25 rDNA copy strains with or without Rpa49. (A) Representative Miller spread from NOY1071 bearing 25 rDNA copies (25 rDNA copies [WT]), BEN20-1a bearing 25 rDNA copies and rpa49Δ (25 rDNA copies [rpa49Δ]), and NOY1064 bearing 190 rDNA copies (190 rDNA copies [WT]). (right) Interpretive tracing of the genes is shown. Polymerases that appear on the gene are shown on the tracing by black dots. (B and C) Cumulative distribution function of the distances between adjacent polymerases in strains NOY1071 (red; 25 rDNA copies [WT]), BEN20-1a (blue; 25 rDNA copies [rpa49Δ]), and NOY1064 (green; 190 rDNA copies [WT]) is shown. Solid lines, empirical measurement; dashed lines, random (B) or simulated (C).

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