Relevance of Shp1 domains for Atg8 interaction. (a) Shp1 domains and generated Shp1 variants. (b) The autophagic rate is measured as in Fig. 1 a with cells chromosomally expressing Shp1 truncations with native promoter. GFP, top; HA antibodies, bottom. (c and e) As in Fig. 4 e, GST-Atg8 beads were incubated with extracts of cells expressing chromosomally (c) or plasmid-encoded (e) truncated HA-tagged Shp1 variants. S, supernatant; W, wash; PD, bound. (d) Autophagic activity of Shp1^-UBA-HA with the CUP1 promoter. Left, induction with 50 µM Cu²⁺; right, medium without exogenous Cu²⁺.