Figure 2.
Figure 2. Normalized fluorescence emission spectra of native aromatic side chains of otoferlin in the presence of 0.1 mM EGTA or 1 mM calcium. 1 µM purified otoferlin fragments were excited at 280 nm and the emission spectra collected from 290 to 380 nm. Representative spectra for each domain under EGTA or calcium conditions are shown. The traces are representative data from four independent trials. FI, fluorescence intensity.

Normalized fluorescence emission spectra of native aromatic side chains of otoferlin in the presence of 0.1 mM EGTA or 1 mM calcium. 1 µM purified otoferlin fragments were excited at 280 nm and the emission spectra collected from 290 to 380 nm. Representative spectra for each domain under EGTA or calcium conditions are shown. The traces are representative data from four independent trials. FI, fluorescence intensity.

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