Figure 6. GPN-induced transient lysosome membrane permeabilization. Lysosomes of RAW264.7 macrophages were loaded with either (A) sulforhodamine or (B) fluorescently labeled dextrans (MW 10,000), and imaged before and after 10 min of GPN treatment. Corresponding DIC images are shown in the insets. (C) The pH of the lysosomes was monitored qualitatively using LysoTracker, which accumulates in acidic intracellular compartments. The dye was added to the cells either before GPN treatment, after 10 min of GPN treatment, or after 10 min of recovery subsequent to 10 min of GPN treatment. Dye accumulation was abrogated when CcA was added to the cells immediately after GPN washout. Representative fields (Bar, 10 µm) are shown along with their corresponding DIC images (insets).
Figure 6.

GPN-induced transient lysosome membrane permeabilization. Lysosomes of RAW264.7 macrophages were loaded with either (A) sulforhodamine or (B) fluorescently labeled dextrans (MW 10,000), and imaged before and after 10 min of GPN treatment. Corresponding DIC images are shown in the insets. (C) The pH of the lysosomes was monitored qualitatively using LysoTracker, which accumulates in acidic intracellular compartments. The dye was added to the cells either before GPN treatment, after 10 min of GPN treatment, or after 10 min of recovery subsequent to 10 min of GPN treatment. Dye accumulation was abrogated when CcA was added to the cells immediately after GPN washout. Representative fields (Bar, 10 µm) are shown along with their corresponding DIC images (insets).

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