Depletion of CHC and/or TACC3 causes comparable mitotic delay and chromosome alignment defect. (A) Western blotting shows the expression levels of CHC and TACC3 in HeLa cells treated with the indicated siRNAs. (B) Bar graph shows mitotic index of HeLa cells treated with the indicated siRNAs (n = 3; >500 cells scored per condition). (C) Box and whisker plot measuring time spent in mitosis of HeLa cells expressing GFP-H2B treated with the indicated siRNAs (>25 cells scored per condition; P < 0.0001). (D) Still images from Video 1 of HeLa cells expressing GFP-H2B treated with the indicated siRNAs are shown. Arrows show chromosome misalignment. Bars, 10 µm. (E) Bar graph shows the percentage of mitotic HeLa cells with a metaphase-like plate and misaligned chromosome after treatment of the indicated siRNAs (n = 3; ∼100 cells per experiment). Error bars indicate mean ± SD.