The CC2 and tail domains inhibit KIF17 motility in trans. (A) Processive motility in vivo. Differentiated CAD cells expressing (1–738)-mCit motor alone (NT, nontransfected control) or together with the indicated Myc-tagged constructs were fixed and stained with an antibody to the Myc tag. Asterisks, cell bodies containing inactive KIF17 motors; arrowheads, neurite tips with accumulation of active KIF17 motors. (B) Quantification of the percentage of cells with (1–738)-mCit tip accumulation from three independent experiments. *, P < 0.05. (C–F) Processive motility in vitro. Single-molecule motility assays were performed for (1–490)-3xmCit motors alone (D) or in the presence of recombinant purified GST (E), GST-CC2 (F), or GST-tail (G) proteins. The speed (red graphs) and run lengths (blue graphs) of the observed events were plotted as histograms. n = number of motility events observed in two independent experiments. Each frame is 50 ms. Bars: (A) 20 µm; (D, E, and G) 2 µm.