Figure 4. Ndel1 phosphorylated at S231 is enriched at the prophase NE. (A) Western blot of COS-7 cells transfected as shown. Cdk5 and cdk1 activity increased pS231Ab signal (lanes 2 and 3). The presence of dncdk1 does not block phosphorylation of S231 by cdk5 (lane 4), but dncdk5 blocks S231 phosphorylation by cdk1 (lane 5). (B) A prophase NE labeled with pS231Ab (green). (C, right) pS231Ab labels the NE in cells treated with nocodazole. (left) Tubulin staining. (D, left) Parasagittal embryonic day 13.5 mouse embryo sections stained with pS231Ab (green) and Hoechst (blue). (right) Only the Hoechst is shown; arrows indicate mitotic cells at the ventricular surface. (E) pS231Ab (left) labels cells with intact LAP2 (right; arrows). Bars: 10 μm (B and C); 20 μm (D); 5 μm (E).
Figure 4.

Ndel1 phosphorylated at S231 is enriched at the prophase NE. (A) Western blot of COS-7 cells transfected as shown. Cdk5 and cdk1 activity increased pS231Ab signal (lanes 2 and 3). The presence of dncdk1 does not block phosphorylation of S231 by cdk5 (lane 4), but dncdk5 blocks S231 phosphorylation by cdk1 (lane 5). (B) A prophase NE labeled with pS231Ab (green). (C, right) pS231Ab labels the NE in cells treated with nocodazole. (left) Tubulin staining. (D, left) Parasagittal embryonic day 13.5 mouse embryo sections stained with pS231Ab (green) and Hoechst (blue). (right) Only the Hoechst is shown; arrows indicate mitotic cells at the ventricular surface. (E) pS231Ab (left) labels cells with intact LAP2 (right; arrows). Bars: 10 μm (B and C); 20 μm (D); 5 μm (E).

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