Localization of GFP-dynein puncta near microtubules in PC12 cell neurites. (A–E) PC12 cells with stable expression of GFP–IC-2C (GFP-Dynein, green) were differentiated with NGF, fixed, and stained with anti-tubulin (red). (A, B, C, and E) GFP-dynein; (D and E) tubulin. (A) PC12 cell with multiple neurites. (B) Enlargement of a region containing discrete dynein puncta indicated by the white box in A. (C–E) Enlargements of the GFP, tubulin, and overlay images, respectively, of the region indicated by the red box in A. (F) Neurite of an untransfected PC12 cell stained with pan dynein antibody 74.1 (Dynein Ab, green). Bars: (A) 10 μm; (B–F) 5 μm.