Figure 9.
Figure 9. RhoGAP7/DLC-1 is preferentially expressed in ERK5-FL ts-v-Src cells, and its overexpression in ERK5−/− ts-v-Src cells can restore podosome formation. (A) ERK5−/− ts-v-Src cells were transfected with a GFP-expressing vector alone (left) or a vector encoding RhoGAP7/DLC-1–GFP fusion. Cells were plated onto glass coverslips and, 24 h later, fixed and stained with rhodamine-phalloidin. Arrows indicate cells expressing fusion protein. (B) Shown is a graphical representation of the mean percentage of cells that had podosomes (n = 3). (C) Src527F cells were transfected with DN-DLC-1–GFP or GFP alone and plated onto glass coverslips. 24 h after plating, cells were fixed and stained with rhodamine-phalloidin and DAPI. Arrows indicate cells expressing GFP or DN-DLC-1–GFP. All images were taken with a 63× objective. Bars, 20 μm. (D) Shown is a graphical representation of the mean percentage of cells that contained podosomes (n = 3). Error bars represent SD.

RhoGAP7/DLC-1 is preferentially expressed in ERK5-FL ts-v-Src cells, and its overexpression in ERK5−/− ts-v-Src cells can restore podosome formation. (A) ERK5−/− ts-v-Src cells were transfected with a GFP-expressing vector alone (left) or a vector encoding RhoGAP7/DLC-1–GFP fusion. Cells were plated onto glass coverslips and, 24 h later, fixed and stained with rhodamine-phalloidin. Arrows indicate cells expressing fusion protein. (B) Shown is a graphical representation of the mean percentage of cells that had podosomes (n = 3). (C) Src527F cells were transfected with DN-DLC-1–GFP or GFP alone and plated onto glass coverslips. 24 h after plating, cells were fixed and stained with rhodamine-phalloidin and DAPI. Arrows indicate cells expressing GFP or DN-DLC-1–GFP. All images were taken with a 63× objective. Bars, 20 μm. (D) Shown is a graphical representation of the mean percentage of cells that contained podosomes (n = 3). Error bars represent SD.

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