Dcp-1 is required for nutrient starvation–induced germarium cell death, and the IAP protein Bruce inhibits germarium and mid-oogenesis cell death. (A) Ovaries were stained with TUNEL (green) to detect DNA fragmentation. Clusters of cysts with TUNEL staining were observed in region 2 in nutrient-deprived w¹¹¹⁸ files. In Dcp-1^Prev flies, fewer TUNEL-positive cysts in region 2 were observed. Under well-fed conditions, numerous TUNEL-positive cysts were observed in Bruce^E81 flies. DAPI staining of nuclei is shown in white. (B) Numerous degenerating stage 8 egg chambers (arrows) with TUNEL-positive staining (green) were observed in well-fed Bruce^E81 flies. DAPI staining of nuclei (white) is shown on the right. Bars: (A) 20 μm; (B) 50 μm.