Figure 4.
Figure 4. Quantification of minor satellite length in wild-type and Dnmt-deficient ES cells. Minor satellite fluorescence distribution in arbitrary units of fluorescence (a.u.f) of wild-type (wt), Dnmt1−/−, and Dnmt3a,3b−/− ES cells at the indicated passage number as determined by Q-FISH using a mouse minor satellite PNA probe (left). Representative images of metaphase spreads of the indicated genotypes are also shown (right). A nonparametric ANOVA test, the Kruskall-wallis test (P < 0.0001), indicated that the observed differences in the centromere fluorescence were not because of coincidences or random sampling. Furthermore, a Dunn's post test indicated that the differences were significant (P < 0.001) for each pair of comparisons. The p-values in the figure refer to comparisons of the different genotypes with wild-type cells. Bars, 7 μm.

Quantification of minor satellite length in wild-type and Dnmt-deficient ES cells. Minor satellite fluorescence distribution in arbitrary units of fluorescence (a.u.f) of wild-type (wt), Dnmt1−/−, and Dnmt3a,3b−/− ES cells at the indicated passage number as determined by Q-FISH using a mouse minor satellite PNA probe (left). Representative images of metaphase spreads of the indicated genotypes are also shown (right). A nonparametric ANOVA test, the Kruskall-wallis test (P < 0.0001), indicated that the observed differences in the centromere fluorescence were not because of coincidences or random sampling. Furthermore, a Dunn's post test indicated that the differences were significant (P < 0.001) for each pair of comparisons. The p-values in the figure refer to comparisons of the different genotypes with wild-type cells. Bars, 7 μm.

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