Localization of cytokinesis proteins in aust spermatocytes. Wild-type and aust mutant spermatocytes were fixed and stained for α-tubulin (green), DNA (blue), and either anillin (A), actin (B), or Fascetto (C, red). Although each protein localizes to the cleavage furrow in wild-type cells, anillin, actin, and Fascetto are not recruited in aust mutants. Bar, 10 μm.